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gradient polyacrylamide gel  (Bio-Rad)


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    Structured Review

    Bio-Rad gradient polyacrylamide gel
    Gradient Polyacrylamide Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 594 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/precast+polyacrylamide+gel/4%E2%80%9315%25+Criterion+TGX+Precast+Midi+Protein+Gel/pm42112903-392-7-10
    Average 96 stars, based on 594 article reviews
    gradient polyacrylamide gel - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Regulation of mitochondrial ROS by C15ORF48 in a basal cell subpopulation contributes to chemotherapy resistance in TNBC
    Article Snippet: Protein concentrations were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). .. Twenty micrograms of protein was heat-denatured, resolved on a 4 to 20% precast polyacrylamide gel (Bio-Rad, 5671094), and analyzed by Western blotting using the primary antibodies C15ORF48 (1:1000, Novus Biologicals, NBP1-98391), vinculin (1:10,000, Abcam, ab129002), caspase-3 (1:1000, Cell Signaling Technology, 9662), and NDUFA4 (1:1000, Possible Missions Inc., NC1704146) and HRP-conjugated secondary antibodies (1:10,000, Thermo Fisher Scientific, 31460). .. Proteins were visualized using SuperSignal West Dura Chemiluminescent Substrate (Thermo Fisher Scientific, 34076) on a G-Box imager (Syngene, USA).

    Article Title: Regulation of mitochondrial ROS by C15ORF48 in a basal cell subpopulation contributes to chemotherapy resistance in TNBC.
    Article Snippet: Protein concentrations were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). .. Twenty micrograms of protein was heat- denatured, resolved on a 4 to 20% precast polyacrylamide gel (Bio- Rad, 5671094), and analyzed by Western blotting using the primary antibodies C15ORF48 (1:1000, Novus Biologicals, NBP198391), vinculin (1:10,000, Abcam, ab129002), caspase- 3 (1:1000, Cell Signaling Technology, 9662), and NDUFA4 (1:1000, Possible Missions Inc., NC1704146) and HRP- conjugated secondary antibodies (1:10,000, Thermo Fisher Scientific, 31460). .. Proteins were visualized using SuperSignal West Dura Chemiluminescent Substrate (Thermo Fisher Scientific, 34076) on a G- Box imager (Syngene, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Regulation of Choroid Plexus Bicarbonate Transporters Following Intraventricular Hemorrhage in Mice
    Article Snippet: .. Proteins were separated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) in a 4%–15% precast polyacrylamide gel (Bio‐Rad, #5671085). .. The electrophoresis was performed at a constant 200 V for approximately 45 min and stained with Gel Code (Thermo Fisher #24590) for 1 h. The gel was de‐stained overnight in Milli‐Q water at 4°C.

    Electrophoresis:

    Article Title: The P2X4 purinergic receptor controls the autophagy-related release of small extracellular vesicles from mammary cancer cells under hypoxia.
    Article Snippet: .. Samples (15 μg) and the Bio-Rad protein markers Precision Plus ProteinTM Dual Color Standards (2 μL) were loaded on to a 4-20% precast polyacrylamide gel (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, France) and electrophorized at 200 V for 45 min (Mini-PROTEAN 3 electrophoresis cell, BioRad, France). ..

    Article Title: The P2X4 purinergic receptor controls the autophagy-related release of small extracellular vesicles from mammary cancer cells under hypoxia
    Article Snippet: .. Samples (15 μg) and the Bio-Rad protein markers Precision Plus ProteinTM Dual Color Standards (2 μL) were loaded on to a 4–20% precast polyacrylamide gel (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, France) and electrophorized at 200 V for 45 min (Mini-PROTEAN 3 electrophoresis cell, BioRad, France). .. The gel was then stained overnight with 5% Coomassie Blue R-350 (PlusOneTM CoomassieTM Blue PhastGelTM Blue R, GE Healthcare, France) in 30% ethanol/10% acetic acid.



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